Antigen

Antigen Class

TFs and others

Antigen

SP1

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

GEO Accession

GSM1010813

antibody antibodydescription

Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1

antibody targetdescription

SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.

antibody vendorid

sc-17824

antibody vendorname

Santa Cruz Biotechnology

antibody

SP1

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

endoderm

cell organism

human

cell sex

M

cell

A549

controlid

SL6021

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL5360,SL5701

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

1 h with 0.02% Ethanol (Myers)

treatment

EtOH_0.02pct

Sequenced DNA Library

library_name

GSM1010813: HudsonAlpha_ChipSeq_A549_SP1_EtOH_0.02pct_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

92693410

Reads aligned (%)

84.2

Duplicates removed (%)

8.1

Number of peaks

43930 (qval < 1E-05)

hg19

Number of total reads

92693410

Reads aligned (%)

83.6

Duplicates removed (%)

9.2

Number of peaks

43643 (qval < 1E-05)